Company: ALLENINSTITUTE
Location: Seattle, WA
Posted on: May 20
Reseach Associate Image-based Assay Develope
The mission of the Allen Institute is to undestand the pinciples that goven life and to advance health. Ou ceative and multi-dimensional teams focus on answeing some of the biggest questions in bioscience. We acceleate foundational eseach, catalyze bold ideas, develop tools and models, and openly shae ou science to make a boad, tansfomational impact on the wold.
The mission of Allen Institute fo Cell Science is to develop a compehensive and holistic appoach to measue, descibe, and model cell states and thei dynamic changes ove time. Ou ultimate goal is to uncove the fundamental pinciples of multiscale, multicellula mophogenesis, including how goups of cells oganize and achieve collective behavios essential fo life. Ou appoach encompasses multi-modal data collection including live 3D timelapse imaging, data analysis, theoy, and pedictions to undestand cell states and cell state tansitions in human induced pluipotent stem cell models. As a division within the Allen Institute, the Allen Institute fo Cell Science uses a team-oiented appoach, focusing on acceleating foundational eseach, developing standads and models, and cultivating new ideas to make a tansfomational impact on science.
We ae looking to hie a Reseach Associate I to contibute to developing obust and quantitative image-based assays of key cellula activities amenable to scale-up and integation into live-cell high-thoughput micoscopy pipelines. The ole includes cell cultuing of undiffeentiated human induced pluipotent stem cells (hiPSC) and fluoescence micoscopy-based imaging of hiPSC, including suppot of imaging automation and pipeline image acquisition. The ole also includes assisting in cell biological quality contol of gene-edited hiPS cell lines geneated by the Institute using westen blotting, immunofluoescence, live-cell micoscopy, and geneal development of cell-based assays. This ole equies coss-team inteaction with the Institutes technical and pogammatic teams to achieve the Institutes matixed, pogammatic objectives.
At the Allen Institute, we believe that science is fo eveyone and should be open to eveyone. We ae dedicated to combating biases and educing baies to STEM caees moe boadly.
We also believe that science is bette when it includes diffeent pespectives and voices. We stive to make the Allen Institute a place whee eveyone feels like they belong and ae empoweed to do thei best wok in a suppotive envionment.
We ae an equal-oppotunity employe and stongly encouage people fom all backgounds to apply fo ou open positions.
Essential Functions
- Assist in developing obust potocols and epoducible image-based assays of cell oganization, dynamics, activities, and function
- Assist in cell cultue opeations fo image-based assay development, woking closely with othe teams at the Institute
- Assist in cell biological quality contol wokflow fo gene-edited hiPS cells
- Assist in micoscope-based data collection and analysis of assay obsevations
- Collaboate with othe teams in the Institute to scale-up potocols into a high thoughput micoscopy pipeline
- Maintain igoous quality contol standads
- Maintain meticulous ecods and wok closely with othe scientists to coodinate complex expeiments
- Adheence to SOPs, GLPs and egulatoy equiements
- Contibute to the implementation of an image management system
- Coodinate with othe pogams in the Institute to ensue seamless integation and shaing of esouces and data
*Note: Reasonable accommodations may be made to enable individuals with disabilities to pefom the essential functions. This desciption eflects managements assignment of essential functions; it does not poscibe o estict the tasks that may be assigned.*
Requied Education and Expeience
- Bachelos degee equied in a biological science, physics, chemisty, o elated field
- 3 months of hands-on cell cultue expeience
- 3 months of hands-on micoscopy expeience
- Expeience pefoming image-based cell biological assays
Pefeed Education and Expeience
- 3 months to 2 yeas of elevant expeience pefeed
- Backgound in cell biology
- Expeience pefoming standad cell biology techniques including westen blotting and immunofluoescence
- Expeience with stem cell tissue cultue
- Poficiency in micoscopy, especially with confocal and spinning disk micoscopes
- Expeience with 3D live-cell imaging techniques
- Ability to define poblems, collect data, establish facts, and daw valid conclusions
- Ability to effectively communicate scientific findings to intenal and extenal goups
- Ability to ead, analyze, and intepet scientific jounal aticles
- Excellent oganization and attention to detail
- Ability to multi-task and eassign pioities as needed of dynamic demands
- Ability to intepet an extensive vaiety of technical instuction
- Ability to wok independently and in a collaboative team envionment
Wok Envionment
- Wok will be conducted in a laboatoy envionment, including potential exposue to hazadous mateials, biohazads, and lases
Physical Demands
- Sitting o standing fo up to 2 hous at a time at a tissue cultue hood, micoscope, o desk
- Occasional lifting of up to 20 pounds
- Fine moto movements in finges/hands to manipulate cultue plates and pipettos
- Push/pull up to 50 lbs on a cat
- Stooping, bending, couching into incubatos, feezes, efigeatos
- Reaching into incubatos, feezes, and efigeatos
Position Type/Expected Hous of Wok
- This ole is cuently woking onsite and is expected to wok onsite fo the majoity of woking hous. The pimay wok location fo this ole is 615 Westlake Ave N., with the flexibility to wok emotely on a limited basis.
Additional Comments
- **Please note, this oppotunity does not offe elocation assistance**
- **Please note, this oppotunity does not offe wok visa sponsoship**
Annualized Salay Range
* Final salay depends on the equied education fo the ole, expeience, level of skills elevant to the ole, and wok location, whee applicable.
Benefits
- Employees (and thei families) ae eligible to enoll in benefits pe eligibility ules outlined in the Allen Institutes Benefits Guide. These benefits include medical, dental, vision, and basic life insuance. Employees ae also eligible to enoll in the Allen Institutes 401k plan. Paid time off is also available as outlined in the Allen Institutes Benefits Guide. Details on the Allen Institutes benefits offeing ae located at the following link to the Benefits Guide: https://alleninstitute.og/caees/benefits.
It is the policy of the Allen Institute to povide equal employment oppotunity (EEO) to all pesons egadless of age, colo, national oigin, citizenship status, physical o mental disability, ace, eligion, ceed, gende, sex, sexual oientation, gende identity and/o expession, genetic infomation, maital status, status with egad to public assistance, vetean status, o any othe chaacteistic potected by fedeal, state o local law. In addition, the Allen Institute will povide easonable accommodations fo qualified individuals with disabilities.
https://discord.gg/GUPad7ETad